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pfuse 631 higg2 fc2  (InvivoGen)


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    Structured Review

    InvivoGen pfuse 631 higg2 fc2
    Pfuse 631 Higg2 Fc2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse+631+higg2+fc2/pFUSE-hIgG2-Fc2/10__1172_slash_jci196788-281-36-38
    Average 94 stars, based on 50 article reviews
    pfuse 631 higg2 fc2 - by Bioz Stars, 2026-09
    94/100 stars

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    Polymerase Chain Reaction:

    Article Title: Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii-derived glutamic acid-maintained Tregs
    Article Snippet: 620 621 Generation of pFUSE-hIgG2-Dectin-1/2-Fc expression plasmids 622 Dectin-1 and Dectin-2 coding sequences were amplified using primers: 623 Dectin-1 F: 5′-CTTCACCTTGGAGGCCCATT-3′; R: 5′-624 TATTTCTGACTTGAAACGAGTTGGG-3′ 625 Dectin-2 F: 5′-CACGAATTCATTTGGCTCCAGCTGCTACCTC-3′; R: 5′-626 GAACCCAATCTTCCAGAAAGATCTGTG-3′ 627 PCR was performed in a 30 μl reaction containing KOD FX Neo (0.15 μl; Toyobo, Japan), 628 primers (1 μl each, 10 mM), dNTPs (6 μl), KOD buffer (15 μl), sterile water (4.85 μl), and 629 cDNA (2 μl). .. Cycling conditions were 94°C for 1 min; 30 cycles of 94°C for 15 s, 56°C for 30 630 s, and 72°C for 30 s; final extension at 72°C for 7 min. PCR products were ligated into pFUSE-631 hIgG2-Fc2 (InvivoGen, San Diego, USA) via EcoRI and BglII (New England Biolabs, USA), 632 transformed into E. coli DH5α (AngYuBio, China), and selected in LB medium (Sangon 633 Biotech, Shanghai, China) with 25 μg/ml Zeocin (MKBio, China). ..

    Transformation Assay:

    Article Title: Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii-derived glutamic acid-maintained Tregs
    Article Snippet: 620 621 Generation of pFUSE-hIgG2-Dectin-1/2-Fc expression plasmids 622 Dectin-1 and Dectin-2 coding sequences were amplified using primers: 623 Dectin-1 F: 5′-CTTCACCTTGGAGGCCCATT-3′; R: 5′-624 TATTTCTGACTTGAAACGAGTTGGG-3′ 625 Dectin-2 F: 5′-CACGAATTCATTTGGCTCCAGCTGCTACCTC-3′; R: 5′-626 GAACCCAATCTTCCAGAAAGATCTGTG-3′ 627 PCR was performed in a 30 μl reaction containing KOD FX Neo (0.15 μl; Toyobo, Japan), 628 primers (1 μl each, 10 mM), dNTPs (6 μl), KOD buffer (15 μl), sterile water (4.85 μl), and 629 cDNA (2 μl). .. Cycling conditions were 94°C for 1 min; 30 cycles of 94°C for 15 s, 56°C for 30 630 s, and 72°C for 30 s; final extension at 72°C for 7 min. PCR products were ligated into pFUSE-631 hIgG2-Fc2 (InvivoGen, San Diego, USA) via EcoRI and BglII (New England Biolabs, USA), 632 transformed into E. coli DH5α (AngYuBio, China), and selected in LB medium (Sangon 633 Biotech, Shanghai, China) with 25 μg/ml Zeocin (MKBio, China). ..



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    94
    InvivoGen pfuse 631 higg2 fc2
    Pfuse 631 Higg2 Fc2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse+631+higg2+fc2/pFUSE-hIgG2-Fc2/10__1172_slash_jci196788-281-36-38
    Average 94 stars, based on 1 article reviews
    pfuse 631 higg2 fc2 - by Bioz Stars, 2026-09
    94/100 stars
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